Synonyms
FLJ33582 antibody, FLJ45739 antibody, MGC40579 antibody, GK5 antibody, putative glycerol kinase 5 antibody, ATP:glycerol 3-phosphotransferase 5 antibody, glycerokinase 5 antibody, GK 5 antibody, glycerol kinase 5 (putative) antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
59 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 9 and 297 of GK5
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded CL1-0 xenograft, using GK5(107-10688) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
107-10688 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.