Rabbit Anti-Human Glucagon (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P01275 |
| Gene Id | 2641 |
| Gene Symbols | GCG |
| Protein Name / Synonyms | Glucagon (C-term) |
| Clonality | Polyclonal |
| Preparation | This Glucagon antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the C-terminal region of human Glucagon. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Apoptosis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Glucagon is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
Specificity
Other Names
GCG; Glucagon; Glicentin; Glicentin-related polypeptide; Oxyntomodulin; Glucagon-like peptide 1; Incretin hormone; Glucagon-like peptide 1(7-37); Glucagon-like peptide 1(7-36); Glucagon-like peptide 2
NCBI Accession #
NP_002045.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
20909
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of Glucagon Antibody (C-term) (Cat# 102-16946) in Jurkat cell line lysates (35ug/lane). GCG (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human colon carcinoma with Glucagon Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of Glucagon Antibody (C-term) (Cat# 102-16946) with pancreas tissue followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
 | Flow cytometric analysis of WiDr cells using Glucagon Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.