ELISA (recommended work dilution= 1-2 ug(1: 1000-2000)), Western Blotting (recommended work dilution= 1:1000)
Immunogen was synthetic active peptide derived from human Glucagon-like Peptide 1 (7-37). This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles.The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (suggesting with sterile PBS) can also be aliquotted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month.
The proglucagon gene is expressed in both pancreatic A cells and intestinal L cells. Tissue-specific posttranslational processing of proglucagon by the prohormone convertase produces the different proglucagon derived peptides (PGDPs) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas the L cells produce several structurally related peptides, including glucagon-like peptide (GLP)-1 and GLP-2, as well as glicentin and oxyntomodulin, which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has recently been found to show intestinal epithelial proliferation. GLP-1 has a major effect of enhancing the release of insulin in response to a glucose stimulus, and coincidentally, suppressing secretion of glucagon. GLP-1 has been shown to potently inhibit several aspects of digestive function, including gastric emptying, gastric secretion and pancreatic secretion.
The rabbit anti-human glucagon-like peptide 1 (7-37) antibody not only specifically bound to synthetic peptide, but also detected target derived from recombinant protein at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Store at -20°C, if not intended for use within a month, Keep it at 4°C and minimize freezing and thawing when use.
- Tang-Christensen, M and et al., Glucagon-like peptide 1(7-36) amide's central inhibition of feeding and peripheral inhibition of drinking are abolished by neonatal monosodium glutamate treatment. Diabetes 47, 530537 (1998).
- McMahon, L.R. & Wellman, P.J. PVN infusion of GLP-1-(736) amide suppresses feeding but does not induce aversion or alter locomotion in rats. Am. J. Physiol. 274, R23R29 (1998).
- Turton, M.D. et al. A role for glucagon-like peptide-1 in the central regulation of feeding. Nature 379, 6972 (1996).