Glycine dehydrogenase antibody [N3C2-2], Internal, 1 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 2731 |
| Protein Name / Synonyms | Glycine dehydrogenase, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
GCE antibody, GCSP antibody, HYGN1 antibody, MGC138198 antibody, MGC138200 antibody, GLDC antibody, "glycine dehydrogenase [decarboxylating], mitochondrial antibody", glycine decarboxylase P-protein antibody, glycine cleavage system P protein antibody, glycine cleavage system protein P antibody, glycine dehydrogenase (decarboxylating) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
113 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 442 and 723 of Glycine dehydrogenase (Uniprot ID# P23378)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal (107-10908) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal (107-10908) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Glycine dehydrogenase(107-10908) antibody at 1:500 dilution.
Glycine dehydrogenase antibody detects Glycine dehydrogenase protein by western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg HepG2 whole cell lysate/extract
5 % SDS-PAGE
Glycine dehydrogenase antibody (107-10908) dilution: 1:500
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg HepG2 whole cell lysate/extract
5 % SDS-PAGE
Glycine dehydrogenase antibody (107-10908) dilution: 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.