Synonyms
GCP170 antibody, MEA-2 antibody, GOLGA3 antibody, Golgin subfamily A member 3 antibody, golgi complex-associated protein of 170 kDa antibody, golgin-160 antibody, golgin-165 antibody, "golgi autoantigen, golgin subfamily a, 3 antibody", Golgi membrane associated protein antibody, male enhanced antigen-2 antibody, Golgi peripheral membrane protein antibody, SY2/SY10 protein antibody, golgin A3 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
167 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1436 and 1498 of GOLGA3 (Uniprot ID# Q08378)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded gastric tissue, using GOLGA3(107-10205) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GOLGA3(107-10205) antibody at 1:200 dilution.

Sample(30 µg of whole cell lysate)
A:A431
B:H1299
5% SDS PAGE
107-10205 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.