Rabbit Anti-Human GTF2I (C-term) Antibody, 400 ul
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P78347 |
| Gene Id | 2969 |
| Gene Symbols | GTF2I |
| Protein Name / Synonyms | GTF2I (C-term) |
| Clonality | Polyclonal |
| Preparation | This GTF2I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 956-985 amino acids from the C-terminal region of human GTF2I. |
| Concentration (lot specific) | 0.330 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
GTF2I is a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23.
Specificity
Predicted Reactivity
Rat
Other Names
GTF2I; BAP135; WBSCR6; General transcription factor II-I; Bruton tyrosine kinase-associated protein 135; SRF-Phox1-interacting protein; Williams-Beuren syndrome chromosomal region 6 protein
NCBI Accession #
NP_001157108.1;NP_001267729.1;NP_001509.3;NP_127492.1;NP_127493.1;NP_127494.1
Other Accession #
Q5U2Y1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
112416
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Fluorescent image of A549 cell stained with GTF2I Antibody (C-term)(Cat# 102-16990/SA091106AV).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).GTF2I immunoreactivity is localized to Nucleus significantly. |
 | GTF2I Antibody (C-term) (Cat# 102-16990) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GTF2I antibody detected the GTF2I protein (arrow). |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with GTF2I Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | GTF2I Antibody (C-term) (Cat# 102-16990) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.