H-Ras antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Gene Id | 3265 |
| Protein Name / Synonyms | H-Ras |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Ras/Rap Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
C-BAS/HAS antibody, C-H-RAS antibody, C-HA-RAS1 antibody, CTLO antibody, H-RASIDX antibody, HAMSV antibody, HRAS1 antibody, K-RAS antibody, N-RAS antibody, RASH1 antibody, HRAS antibody, transforming protein p21 antibody, Ha-Ras1 proto-oncoprotein antibody, H-Ras-1 antibody, GTPase HRas antibody, c-has/bas p21 protein antibody, transformation gene: oncogene HAMSV antibody, p19 H-RasIDX protein antibody, GTP- and GDP-binding peptide B antibody, p21ras antibody, Ras family small GTP binding protein H-Ras antibody, ha-Ras antibody, c-ras-Ki-2 activated oncogene antibody, v-Ha-ras Harvey rat sarcoma viral oncogene homolog antibody
Specificity
Species Reactivity
Human, Rat
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
21 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 111 and 189 of H-Ras (Uniprot ID# P01112)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
H-Ras antibody detects HRAS protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
H-Ras antibody (107-11169) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
H-Ras antibody (107-11169) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-Ras (107-11169) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using H-Ras (107-11169) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
C: Molt-4
12% SDS PAGE
107-11169 diluted at 1:1000
A: Hela
B: Hep G2
C: Molt-4
12% SDS PAGE
107-11169 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.