Rabbit Anti-Human HDAC1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13547 |
| Gene Id | 3065 |
| Gene Symbols | HDAC1 |
| Protein Name / Synonyms | HDAC1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This HDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 449-482 amino acids from the C-terminal region of human HDAC1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Specificity
Predicted Reactivity
Mouse
Other Names
HDAC1; RPD3L1; Histone deacetylase 1
NCBI Accession #
NP_004955.2
Other Accession #
O09106
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
55103
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
 | Fluorescent image of Hela cell stained with HDAC1 Antibody (C-term)(Cat# 102-10902/SH040527D).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).HDAC1 immunoreactivity is localized to Nucleus significantly. |
 | The anti-HDAC1 Pab (Cat# 102-10902) is used in Western blot to detect HDAC1 in ZR-75-1 cell lysate. |
 | The anti-HDAC1 Pab (Cat# 102-10902) was used in Western Blot to detect HDAC1 in HEK293 cells. Knockdown of HDAC1 using siRNA against HDAC1 showed a significant decrease of HDAC1 protein using this anti-HDAC1 Pab in HEK293 cells. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.