HICE1 antibody, 0.17 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 93323 |
| Protein Name / Synonyms | HICE1 |
| Concentration (lot specific) | 0.17 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DGT4 antibody, HICE1 antibody, MGC20533 antibody, NY-SAR-48 antibody, HAUS8 antibody, "HEC1/NDC80 interacting, centrosome associated 1 antibody", Hec1-interacting and centrosome-associated 1 antibody, sarcoma antigen NY-SAR-48 antibody, HEC1/NDC80-interacting centrosome-associated protein 1 antibody, HAUS augmin-like complex subunit 8 antibody, "HAUS augmin-like complex, subunit 8 antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
45 kDa
Immunogen
Recombinant protein corresponding to a region within amino acids 166 and 360 of human HICE1 (Uniprot # Q9BT25)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:50-1:500 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of human osteosarcoma cell line U2OS, using HICE1(107-10237) antibody at 1:50-1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (107-10237) antibody at 1:50 dilution.
Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using HICE1 (107-10237) antibody at 1:50 dilution.
IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components HAUS6, HAUS2 and HAUS1 in U2OS cells.
Sample
A: His-Hice1 (2x)
B: His-Hice1 (1x)
7.5% SDS PAGE
107-10237 diluted at 1:500
A: His-Hice1 (2x)
B: His-Hice1 (1x)
7.5% SDS PAGE
107-10237 diluted at 1:500
WB to detect cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image), using 107-10237 at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) at 1:5000 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.