This HIST1H2BJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the Central region of human HIST1H2BJ.
Host Species
Rabbit
Clonality
Polyclonal
Species
Human
Introduction
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq].
Specificity
Predicted Reactivity
Mouse, Rat, Zebrafish, Bovine, Chicken, Monkey, Xenopus
Other Names
HIST1H2BJ; H2BFR; Histone H2B type 1-J; Histone H2B.1; Histone H2B.r
HIST1H2BJ antibody (Center) (Cat# 102-10774) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2BJ antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
HIST1H2BJ Antibody (Center) (Cat# 102-10774) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
HIST1H2BJ Antibody (Center) (Cat# 102-10774) western blot analysis in CEM,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST1H2BJ antibody detected the HIST1H2BJ protein (arrow).
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
