Rabbit Anti-Human HMGA1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P17096 |
| Gene Id | 3159 |
| Gene Symbols | HMGA1 |
| Protein Name / Synonyms | HMGA1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the C-terminal region of human HMGA1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.
Specificity
Predicted Reactivity
Mouse, Rat, Hamster
Other Names
HMGA1; HMGIY; High mobility group protein HMG-I/HMG-Y; High mobility group AT-hook protein 1; High mobility group protein R
NCBI Accession #
NP_002122.1;NP_665906.1;NP_665908.1;NP_665909.1;NP_665910.1;NP_665912.1
Other Accession #
Q8K585; P17095; Q9QXP3
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
11676
Recommended Dilutions
IF: 1:10-50; WB: 1:1000; FC: 1:10-50
 | Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term)(Cat# 102-18144).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).HMGA1 immunoreactivity is localized to Nucleus significantly. |
 | HMGA1 Antibody (C-term) (Cat# 102-18144) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow). |
 | HMGA1 Antibody (C-term) (Cat# 102-18144) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.