HPRT antibody, 0.95 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 3251 |
| Protein Name / Synonyms | HPRT |
| Concentration (lot specific) | 0.95 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
HGPRT antibody, HPRT antibody, HPRT1 antibody, hypoxanthine-guanine phosphoribosyltransferase antibody, HGPRTase antibody, hypoxanthine phosphoribosyltransferase 1 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
25 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 23 and 218 of HPRT (Uniprot ID# P00492)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using HPRT(107-11068) antibody at 1:100 dilution.
HPRT antibody detects HPRT1 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
12 % SDS-PAGE
HPRT antibody (107-11068) dilution: 1:500
A. 50 µg mouse liver lysate/extract
12 % SDS-PAGE
HPRT antibody (107-11068) dilution: 1:500
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HPRT(107-11068) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: Molt-4
B: Raji
12% SDS PAGE
107-11068 diluted at 1:1000
A: Molt-4
B: Raji
12% SDS PAGE
107-11068 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.