Rabbit Anti-Human HPRT1 (C-term) Antibody, 400 ul
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P00492 |
| Gene Id | 3251 |
| Gene Symbols | HPRT1 |
| Protein Name / Synonyms | HPRT1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This HPRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-178 amino acids from the C-terminal region of human HPRT1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Specificity
Predicted Reactivity
Monkey
Other Names
HPRT1; HPRT; Hypoxanthine-guanine phosphoribosyltransferase
NCBI Accession #
NP_000185.1
Other Accession #
Q6LDD9
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
24579
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of HPRT1 antibody (C-term) (Cat# 102-16915) in Hela cell line lysates (35ug/lane). HPRT1 (arrow) was detected using the purified Pab. |
 | Western blot analysis of HPRT1 (arrow) using rabbit polyclonal HPRT1 Antibody (C-term) (Cat# 102-16915). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HPRT1 gene. |
 | HPRT1 Antibody (C-term) (Cat# 102-16915) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HPRT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | HPRT1 Antibody (C-term) (Cat# 102-16915) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.