Rabbit Anti-Human HPRT1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P00492 |
| Gene Id | 3251 |
| Gene Symbols | HPRT1 |
| Protein Name / Synonyms | HPRT1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This HPRT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-94 amino acids from the N-terminal region of human HPRT1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Specificity
Predicted Reactivity
Mouse, Rat, Hamster, Pig, Monkey
Other Names
HPRT1; HPRT; Hypoxanthine-guanine phosphoribosyltransferase
NCBI Accession #
NP_000185.1
Other Accession #
P27605; Q45FY6; P00493; Q6LDD9; P00494
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
24579
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of HPRT1 (arrow) using rabbit polyclonal HPRT1 Antibody (N-term) (Cat# 102-16914). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HPRT1 gene. |
 | HPRT1 Antibody (N-term) (Cat# 102-16914)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HPRT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | HPRT1 Antibody (N-term) (Cat# 102-16914) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.