HSD11B1 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Ovine (sheep) |
| Host Species | Rabbit |
| Gene Id | 3290 |
| Protein Name / Synonyms | HSD11B1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), IHC (Frozen sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
11-DH antibody, 11-beta-HSD1 antibody, HDL antibody, HSD11 antibody, HSD11B antibody, HSD11L antibody, MGC13539 antibody, SDR26C1 antibody, HSD11B1 antibody, corticosteroid 11-beta-dehydrogenase isozyme 1 antibody, short chain dehydrogenase/reductase family 26C, member 1 antibody, hydroxysteroid (11-beta) dehydrogenase 1 antibody
Specificity
Species Reactivity
Human, Sheep
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
32 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 232 and 292 of HSD11B1
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),IHC (Frozen sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000.IHC-Fr:Assay dependent. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
HSD11B1 antibody detects HSD11B1 protein at cytosol on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
HSD11B1 antibody (107-10466) dilution: 1:500.
Sample: Paraffin-embedded hepatoma.
HSD11B1 antibody (107-10466) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HSD11B1(107-10466) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
12% SDS PAGE
107-10466 diluted at 1:1000
A: Hep G2
12% SDS PAGE
107-10466 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.