HSD17B4 antibody, 0.66 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 3295 |
| Protein Name / Synonyms | HSD17B4 |
| Concentration (lot specific) | 0.66 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DBP antibody, MFE-2 antibody, MPF-2 antibody, SDR8C1 antibody, HSD17B4 antibody, short chain dehydrogenase/reductase family 8C, member 1 antibody, peroxisomal multifunctional protein 2 antibody, 17beta-estradiol dehydrogenase type IV antibody, D-3-hydroxyacyl-CoA dehydratase antibody, 17-beta-HSD IV antibody, beta-keto-reductase antibody, multifunctional protein 2 antibody, 17-beta-HSD 4 antibody, peroxisomal multifunctional enzyme type 2 antibody, beta-hydroxyacyl dehydrogenase antibody, 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody, 17-beta-hydroxysteroid dehydrogenase 4 antibody, D-bifunctional protein, peroxisomal antibody, hydroxysteroid (17-beta) dehydrogenase 4 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
80 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 18 and 261 of HSD17B4 (Uniprot ID# P51659)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
HSD17B4 antibody detects HSD17B4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: HSD17B4 protein stained by HSD17B4 antibody (107-11145) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: HSD17B4 protein stained by HSD17B4 antibody (107-11145) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded OVCA, using HSD17B4(107-11145) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-11145 diluted at 1:1000
A: Mouse brain
7.5% SDS PAGE
107-11145 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
107-11145 diluted at 1:1000
A: Hela
7.5% SDS PAGE
107-11145 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.