Rabbit Anti-Human IL-8 / CXCL8 [+Biotin]
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Anti-IL-8 / CXCL8 Antibody, Biotinylated
| Size | 50 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | This product is specific for human IL-8 (Interleukin-8), a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. |
| Host Species | Rabbit |
| Protein Name / Synonyms | IL-8 / CXCL8 |
| Preparation | Antisera to human IL-8 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography. |
| Concentration (lot specific) | IgG concentration 0.1mg/ml after reconstitution |
| Recommended Applications | ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting |
| Reconstitution | Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.. For long term storage the addition of 0.09% sodium azide is recommended. |
| Research Area | Inflammation, Chemokine Biology |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Prior to reconstitution store at +4 °C. Following reconstitution store at -20 °C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Antisera to human IL-8 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
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Anti-IL-8 / CXCL8 Antibody, Biotinylated