IL1 Receptor antagonist antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 3557 |
| Protein Name / Synonyms | IL1 Receptor antagonist |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DIRA antibody, ICIL-1RA antibody, IL-1RN antibody, IL-1ra antibody, IL-1ra3 antibody, IL1F3 antibody, IL1RA antibody, IRAP antibody, MGC10430 antibody, MVCD4 antibody, IL1RN antibody, IL1 inhibitor antibody, IL1RN (IL1F3) antibody, interleukin-1 receptor antagonist protein antibody, intracellular IL-1 receptor antagonist type II antibody, type II interleukin-1 receptor antagonist antibody, intracellular interleukin-1 receptor antagonist (icIL-1ra) antibody, interleukin 1 receptor antagonist antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
20 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 177 of IL1ra antagonist (Uniprot ID# P18510)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using IL1 Receptor antagonist(107-10628) antibody at 1:500 dilution.
IL1 Receptor antagonist antibody detects IL1RN protein by Western blot analysis.
A. 30 µg Raji whole cell lysate/extract
15 % SDS-PAGE
IL1 Receptor antagonist antibody (107-10628) dilution: 1:1000
A. 30 µg Raji whole cell lysate/extract
15 % SDS-PAGE
IL1 Receptor antagonist antibody (107-10628) dilution: 1:1000
IL1 Receptor antagonist antibody detects IL1RN protein by Western blot analysis.
A. 30 µg Neuro2A whole lysate/extract
B. 30 µg C8D30 whole cell lysate/extract
C. 30 µg NIH-3T3 whole cell lysate/extract
D. 30 µg Raw264.7 whole cell lysate/extract
E. 30 µg C2C12 whole cell lysate/extract
15 % SDS-PAGE
IL1 Receptor antagonist antibody (107-10628) dilution: 1:1000
A. 30 µg Neuro2A whole lysate/extract
B. 30 µg C8D30 whole cell lysate/extract
C. 30 µg NIH-3T3 whole cell lysate/extract
D. 30 µg Raw264.7 whole cell lysate/extract
E. 30 µg C2C12 whole cell lysate/extract
15 % SDS-PAGE
IL1 Receptor antagonist antibody (107-10628) dilution: 1:1000
IL1 Receptor antagonist antibody detects IL1RN protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
15 % SDS-PAGE
IL1 Receptor antagonist antibody (107-10628) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
15 % SDS-PAGE
IL1 Receptor antagonist antibody (107-10628) dilution: 1:1000
Immunofluorescence analysis of methanol-fixed HeLa, using IL1ra antagonist (107-10628) antibody at 1:200 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.