Rabbit Anti-Human IL1B (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P01584 |
| Gene Id | 3553 |
| Gene Symbols | IL1B |
| Protein Name / Synonyms | IL1B (Center) |
| Clonality | Polyclonal |
| Preparation | This IL1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-174 amino acids from the Central region of human IL1B. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity.
Specificity
Predicted Reactivity
Monkey, Rabbit
Other Names
IL1B; IL1F2; Interleukin-1 beta; Catabolin
NCBI Accession #
NP_000567.1
Other Accession #
P14628; P79182
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
30748
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of IL1B Antibody (Center) (Cat# 102-17904) in NCI-H460 cell line lysates (35ug/lane). IL1B (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma with IL1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of NCI-H292 cells using IL1B Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.