Rabbit Anti-Human ILK (S246) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13418 |
| Gene Id | 3611 |
| Gene Symbols | ILK |
| Protein Name / Synonyms | ILK (S246) |
| Clonality | Polyclonal |
| Preparation | This ILK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from human ILK. |
| Concentration (lot specific) | 0.430 mg/ml |
| Recommended Applications | IHC-P, Western Blotting |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK is a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine
Other Names
ILK; ILK1; ILK2; Integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; ILK-1; ILK-2; p59ILK
NCBI Accession #
NP_001014794.1;NP_001014795.1;NP_001265370.1;NP_004508.1
Other Accession #
Q99J82; O55222; Q3SWY2; NP_004508
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
51419
Recommended Dilutions
IHC: 1:50-100; WB: 1:1000
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ILK Antibody (S246) (Cat# 102-17518), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Western blot analysis of anti-ILK Antibody (S246) (Cat# 102-17518) in Hela cell line lysates (35ug/lane). ILK(arrow) was detected using the purified Pab. |
 | Western blot analysis of ILK (arrow) using rabbit polyclonal ILK Antibody (pS246) (Cat# 102-17518). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ILK gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.