Importin 7 antibody [C3], C-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 10527 |
| Protein Name / Synonyms | Importin 7, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FLJ14581 antibody, Imp7 antibody, MGC138673 antibody, RANBP7 antibody, IPO7 antibody, importin-7 antibody, RAN-binding protein 7 antibody, importin 7 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
120 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 976 and 1038 of Importin 7 (Uniprot ID# O95373)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Importin 7 antibody [C3], C-term detects IPO7 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IPO7 protein stained by Importin 7 antibody [C3], C-term (107-10618) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: IPO7 protein stained by Importin 7 antibody [C3], C-term (107-10618) diluted at 1:500.
Blue: Hoechst 33343 staining.
Immunohistochemical analysis of paraffin-embedded Huh7 xenograft, using Importin 7(107-10618) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
107-10618 diluted at 1:1000
A: JurKat
7.5% SDS PAGE
107-10618 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.