Synonyms
5PTase antibody, MGC65156 antibody, MGC71303 antibody, INPP5B antibody, "type II inositol-1,4,5-trisphosphate 5-phosphatase antibody", phosphoinositide 5-phosphatase antibody, 75 kDa inositol polyphosphate-5-phosphatase antibody, "inositol polyphosphate-5-phosphatase, 75kDa antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
113 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 699 and 949 of INPP5B (Uniprot ID# P32019)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using INPP5B(107-11026) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
107-11026 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.