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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q96A47 |
| Gene Id | 64843 |
| Gene Symbols | ISL2 |
| Protein Name / Synonyms | ISL2 (N-term) |
| Clonality | Polyclonal |
| Preparation | This ISL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ISL2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IF, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
ISL2 is a transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways (By similarity).
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
ISL2; Insulin gene enhancer protein ISL-2
NCBI Accession #
NP_665804.1
Other Accession #
P50480; Q9CXV0; NP_665804.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
39768
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
 | Fluorescent image of HUVEC cell stained with ISL2 Antibody (N-term)(Cat# 102-13031).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ISL2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).ISL2 immunoreactivity is localized to Nucleus significantly. |
 | ISL2 Antibody (N-term) (Cat# 102-13031) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ISL2 antibody detected the ISL2 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.