Rabbit Anti-Human ITPA (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9BY32 |
| Gene Id | 3704 |
| Gene Symbols | ITPA |
| Protein Name / Synonyms | ITPA (N-term) |
| Clonality | Polyclonal |
| Preparation | This ITPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human ITPA. |
| Concentration (lot specific) | 0.470 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
ITPA hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. The encoded protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency. Two transcript variants encoding two different isoforms have been found for this gene.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Chicken, Xenopus
Other Names
ITPA; C20orf37; Inosine triphosphate pyrophosphatase; Non-canonical purine NTP pyrophosphatase; Non-standard purine NTP pyrophosphatase; Nucleoside-triphosphate diphosphatase; Nucleoside-triphosphate pyrophosphatase; Putative oncogene protein hlc14-06-p
NCBI Accession #
NP_001254552.1;NP_258412.1;NP_852470.1
Other Accession #
Q2NLA8; D3ZW55; Q9D892; F1NLH9; Q2KIC5
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
21446
Recommended Dilutions
IF: 1:25; WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Immunofluorescent analysis of Hela cells, using ITPA Antibody (N-term)(Cat# 102-15804). 102-15804 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red). |
 | Western blot analysis of ITPA Antibody (N-term) (Cat# 102-15804) in A375 cell line lysates (35ug/lane). ITPA (arrow) was detected using the purified Pab. |
 | ITPA Antibody (N-term) (Cat# 102-15804) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITPA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | ITPA Antibody (N-term) (Cat# 102-15804) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.