This JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human JMJD3.
Host Species
Rabbit
Clonality
Polyclonal
Species
Human
Introduction
Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
Specificity
Predicted Reactivity
Mouse
Other Names
KDM6B; JMJD3; KIAA0346; Lysine-specific demethylase 6B; JmjC domain-containing protein 3; Jumonji domain-containing protein 3; Lysine demethylase 6B
NCBI Accession #
NP_001073893.1
Other Accession #
Q5NCY0
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
176632
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50
Western blot analysis of anti-JMJD3 (N-term) Pab (102-10599) in Hela cell line lysates. JMJD3 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human brain tissue reacted with JMJD3 (N-term) (Cat# 102-10599), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of JMJD3 Antibody (N-term) (Cat# 102-10599) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
