Rabbit Anti-Human JUN (Center T93) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P05412 |
| Gene Id | 3725 |
| Gene Symbols | JUN |
| Protein Name / Synonyms | JUN (Center T93) |
| Clonality | Polyclonal |
| Preparation | This JUN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-98 amino acids from the Central region of human JUN. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Pig
Other Names
JUN; Transcription factor AP-1; Activator protein 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39
NCBI Accession #
NP_002219.1
Other Accession #
P17325; P56432; P05627; O77627; NP_002219.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
35676
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | JUN Antibody (Center T93) (Cat# 102-14055) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the JUN antibody detected the JUN protein (arrow). |
 | Fluorescent confocal image of U251 cell stained with JUN Antibody (Center T93)(Cat# 102-14055).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with JUN primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). JUN immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.