Ku80 antibody [N3C2], Internal, 1.01 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 7520 |
| Protein Name / Synonyms | Ku80, Internal |
| Concentration (lot specific) | 1.01 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FLJ39089 antibody, KARP-1 antibody, KARP1 antibody, KU80 antibody, KUB2 antibody, Ku86 antibody, NFIV antibody, XRCC5 antibody, ATP-dependent DNA helicase II 80 kDa subunit antibody, CTC85 antibody, TLAA antibody, Ku86 autoantigen related protein 1 antibody, nuclear factor IV antibody, 86 kDa subunit of Ku antigen antibody, thyroid-lupus autoantigen antibody, Ku autoantigen, 80kDa antibody, ATP-dependent DNA helicase 2 subunit 2 antibody, DNA repair protein XRCC5 antibody, X-ray repair cross-complementing protein 5 antibody, CTC box-binding factor 85 kDa subunit antibody, CTCBF antibody, lupus Ku autoantigen protein p86 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
83 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 315 and 593 of Ku80 (Uniprot ID# P13010)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Ku80(XRCC5) antibody immunoprecipitates Ku80 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of Ku80 protein by 2.5 µg of Ku80 antibody (107-10881) 7.5% SDS-PAGE The immunoprecipitated Ku80 protein was detected by Ku80 antibody (107-10881) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Ku80 antibody [N3C2], Internal detects XRCC5 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Ku80 antibody [N3C2], Internal (107-10881) dilution: 1:1000
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
Ku80 antibody [N3C2], Internal (107-10881) dilution: 1:1000
Immunofluorescence analysis of methanol-fixed HeLa, using Ku80 (XRCC5)(107-10881) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Ku80 (XRCC5)(107-10881) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.