Lamin B1 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 4001 |
| Protein Name / Synonyms | Lamin B1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ADLD antibody, LMN antibody, LMN2 antibody, LMNB antibody, MGC111419 antibody, LMNB1 antibody, lamin-B1 antibody, lamin B1 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
66 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 289 and 574 of Lamin B1 (Uniprot ID# P20700)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Colon ca, using Lamin B1 antibody(107-10381) antibody at 1:250 dilution.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
107-10381 diluted at 1:5000
A: NIH-3T3
7.5% SDS PAGE
107-10381 diluted at 1:5000
Sample(30 ug whole cell lysate)
A:A431
B:H1299
C:HeLa S3
7.5% SDS PAGE
107-10381 diluted at 1:5000
A:A431
B:H1299
C:HeLa S3
7.5% SDS PAGE
107-10381 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.