Laminin beta 3 antibody [C1C2], Internal, 0.73 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 3914 |
| Protein Name / Synonyms | Laminin beta 3, Internal |
| Concentration (lot specific) | 0.46 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Cell Adhesion |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
BM600-125KDA antibody, FLJ99565 antibody, LAM5 antibody, LAMNB1 antibody, LAMB3 antibody, kalinin B1 chain antibody, laminin B1k chain antibody, nicein-125kDa antibody, "laminin, beta 3 (nicein (125kD), kalinin (140kD), BM600 (125kD)) antibody", kalinin subunit beta antibody, laminin subunit beta-3 antibody, laminin S B3 chain antibody, epiligrin subunit bata antibody, kalinin-140kDa antibody, nicein subunit beta antibody, laminin-5 subunit beta antibody, "laminin, beta 3 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
130 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 644 and 960 of Laminin beta 3 (Uniprot ID# Q13751)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using Laminin beta 3(107-10402) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Lung ca, using Laminin beta 3(107-10402) antibody at 1:250 dilution.
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
Laminin beta-3 antibody
107-10402 diluted at 1:1000
A: A431
7.5% SDS PAGE
Laminin beta-3 antibody
107-10402 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
107-10402 diluted at 1:1000
A:NIH-3T3
7.5% SDS PAGE
107-10402 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.