Synonyms
KIAA0028 antibody, LEURS antibody, MGC26121 antibody, LARS2 antibody, "probable leucyl-tRNA synthetase, mitochondrial antibody", "leucine tRNA ligase 2, mitocondrial antibody", leucine translase antibody, "leucyl-tRNA synthetase 2, mitochondrial antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
102 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 181 and 458 of LARS2 (Uniprot ID# Q15031)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A431, using LARS2(107-10896) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LARS2(107-10896) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-10896 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.