LDB1 antibody [N2C3], 0.93 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 8861 |
| Protein Name / Synonyms | LDB1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CLIM2 antibody, NLI antibody, LDB1 antibody, CLIM-2 antibody, hLdb1 antibody, LDB-1 antibody, LIM domain-binding factor-1 antibody, nuclear LIM interactor antibody, LIM domain-binding factor CLIM2 antibody, carboxyl-terminal LIM domain-binding protein 2 antibody, carboxy terminal LIM domain protein 2 antibody, LIM domain-binding protein 1 antibody, LIM domain binding 1 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
1X PBS, 20% Glycerol (pH7), 0.025% Proclin 300 was added as a preservative.
Calculated Molecular Weight
47 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 86 and 411 of LDB1 (Uniprot ID# Q86U70)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
LDB1antibody immunoprecipitates LDB1 protein in IP experiments.
IP Sample: 1000 µg 293T whole cell lysate/extract
A. 20 µg 293T whole cell lysate/extract
B. Control with 2.5 µg of preimmune rabbit IgG
C. Immunoprecipitation of LDB1 protein by 2.5 µg of LDB1 antibody (107-10458)
12% SDS-PAGE
The immunoprecipitated LDB1 protein was detected by LDB1antibody (107-10458) diluted at 1:1000.
EasyBlot anti-rabbit IgG was used as a secondary reagent.
IP Sample: 1000 µg 293T whole cell lysate/extract
A. 20 µg 293T whole cell lysate/extract
B. Control with 2.5 µg of preimmune rabbit IgG
C. Immunoprecipitation of LDB1 protein by 2.5 µg of LDB1 antibody (107-10458)
12% SDS-PAGE
The immunoprecipitated LDB1 protein was detected by LDB1antibody (107-10458) diluted at 1:1000.
EasyBlot anti-rabbit IgG was used as a secondary reagent.
Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
107-10458 diluted at 1:1000
A: Mouse brain
10% SDS PAGE
107-10458 diluted at 1:1000
LDB1 antibody [N2C3] detects LDB1 protein by western blot analysis.
A. 30 µg 293T whole cell extract
B. 30 µg A431 whole cell extract
C. 30 µg HeLa whole cell extract
D. 30 µg HepG2 whole cell extract
10 % SDS-PAGE
LDB1 antibody [N2C3] (107-10458) dilution: 1:1000
A. 30 µg 293T whole cell extract
B. 30 µg A431 whole cell extract
C. 30 µg HeLa whole cell extract
D. 30 µg HepG2 whole cell extract
10 % SDS-PAGE
LDB1 antibody [N2C3] (107-10458) dilution: 1:1000
LDB1 antibody [N2C3] detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
LDB1 antibody [N2C3] (107-10458) dilution: 1:500.
Sample: Paraffin-embedded mouse muscle.
LDB1 antibody [N2C3] (107-10458) dilution: 1:500.
LDB1 antibody [N2C3] detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
LDB1 antibody [N2C3] (107-10458) dilution: 1:500.
Sample: Paraffin-embedded rat middle brain.
LDB1 antibody [N2C3] (107-10458) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using LDB1(107-10458) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using LDB1(107-10458) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.