Rabbit Anti-Human LFNG (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q8NES3 |
| Gene Id | 3955 |
| Gene Symbols | LFNG |
| Protein Name / Synonyms | LFNG (Center) |
| Clonality | Polyclonal |
| Preparation | This LFNG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-114 amino acids from the Central region of human LFNG. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
LFNG belongs to evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This protein is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150).
Specificity
Other Names
LFNG; Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
NCBI Accession #
NP_001035257.1;NP_001035258.1;NP_001159827.1;NP_002295.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
41773
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | LFNG Antibody (Center) (Cat# 102-18298) western blot analysis in HL-60,K562,Hela cell line lysates (35ug/lane).This demonstrates the LFNG antibody detected the LFNG protein (arrow). |
 | LFNG Antibody (Center)(Cat# 102-18298) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LFNG Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | LFNG Antibody (Center) (Cat# 102-18298) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.