LIMCH1 antibody [C2C3], C-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 22998 |
| Protein Name / Synonyms | LIMCH1, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp434I0312 antibody, DKFZp686A01247 antibody, DKFZp686B2470 antibody, DKFZp686G18243 antibody, DKFZp686G2094 antibody, DKFZp781C1754 antibody, DKFZp781I1455 antibody, LIMCH1A antibody, LMO7B antibody, MGC72127 antibody, LIMCH1 antibody, LIM and calponin homology domains-containing protein 1 antibody, LIM and calponin homology domains 1 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
122 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 734 and 1007 (Q9UPQ0) of LIMCH1
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LIMCH1(107-10789) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10789 diluted at 1:2000
A: Mouse brain
5% SDS PAGE
107-10789 diluted at 1:2000
Sample (30µg whole cell lysate)
A:H1299
7.5% SDS PAGE
107-10789 diluted at 1:3000
A:H1299
7.5% SDS PAGE
107-10789 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.