MAD2L1 antibody [C2C3], C-Terminus, 1 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 4085 |
| Protein Name / Synonyms | MAD2L1, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
HSMAD2 antibody, MAD2 antibody, MAD2L1 antibody, "MAD2 (mitotic arrest deficient, yeast, homolog)-like 1 antibody", MAD2-like protein 1 antibody, mitotic spindle assembly checkpoint protein MAD2A antibody, "mitotic arrest deficient, yeast, homolog-like 1 antibody", mitotic arrest deficient 2-like protein 1 antibody, MAD2 mitotic arrest deficient-like 1 (yeast) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
24 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 142 and 205 of MAD2L1 (Uniprot ID# Q13257)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: Use at an assay dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
MAD2L1 antibody [C2C3], C-term (107-10473) dilution: 1:500.
Sample: Paraffin-embedded rat fore brain.
MAD2L1 antibody [C2C3], C-term (107-10473) dilution: 1:500.
Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(107-10473) antibody at 1:1000 dilution.
Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Mad2L1(107-10473) antibody at 1:500 dilution.
Sample (30µg whole cell lysate)
A:Hep G2
B:MOLT4
C:Raji
12% SDS PAGE
107-10473 diluted at 1:1000
A:Hep G2
B:MOLT4
C:Raji
12% SDS PAGE
107-10473 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.