Synonyms
CT1.11 antibody, MAGE-11 antibody, MAGE11 antibody, MAGEA-11 antibody, MGC10511 antibody, MAGEA11 antibody, "cancer/testis antigen family 1, member 11 antibody", MAGE-11 antigen antibody, cancer/testis antigen 1.11 antibody, melanoma-associated antigen 11 antibody, "melanoma antigen family A, 11 antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
48 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 78 and 173 of MAGEA11 (Uniprot ID# P43364)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using MAGEA11(107-10918) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft , using MAGEA11(107-10918) antibody at 1:500 dilution.

Sample(30 ug whole cell lysate)
A:A431
B:H1299
10% SDS PAGE
107-10918 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.