Rabbit Anti-Human MAPE (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P78395 |
| Gene Id | 23532 |
| Gene Symbols | PRAME |
| Protein Name / Synonyms | MAPE (C-term) |
| Clonality | Polyclonal |
| Preparation | This MAPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human MAPE. |
| Concentration (lot specific) | 2.000 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias.
Specificity
Other Names
PRAME; MAPE; OIP4; Melanoma antigen preferentially expressed in tumors; Opa-interacting protein 4; Preferentially expressed antigen of melanoma
NCBI Accession #
NP_006106.1;NP_996836.1;NP_996837.1;NP_996838.1;NP_996839.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
57890
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of MAPE antibody (C-term) (Cat# 102-16908) in K562 cell line lysates (35ug/lane). MAPE (arrow) was detected using the purified Pab. |
 | MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | MAPE Antibody (C-term) (Cat# 102-16908) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.