ME1 antibody, 0.87 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 4199 |
| Protein Name / Synonyms | ME1 |
| Concentration (lot specific) | 0.87 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
HUMNDME antibody, MES antibody, ME1 antibody, pyruvic-malic carboxylase antibody, "Malic enzyme, cytoplasmic antibody", "malic enzyme 1, soluble antibody", NADP-dependent malic enzyme antibody, malate dehydrogenase antibody, NADP-ME antibody, "malic enzyme 1, NADP(+)-dependent, cytosolic antibody"
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
64 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 161 and 519 of (Uniprot ID# P48163)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
ME1 antibody detects ME1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
ME1 antibody (107-10435) dilution: 1:500.
Sample: Paraffin-embedded human ovarian carcinoma.
ME1 antibody (107-10435) dilution: 1:500.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
107-10435 diluted at 1:1000
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
107-10435 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: PC-12
7.5% SDS PAGE
107-10435 diluted at 1:1000
A: PC-12
7.5% SDS PAGE
107-10435 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
107-10435 diluted at 1:1000
A: A431
7.5% SDS PAGE
107-10435 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ME1(107-10435) antibody at 1:200 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.