Rabbit Anti-Human MMP14 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P50281 |
| Gene Id | 4323 |
| Gene Symbols | MMP14 |
| Protein Name / Synonyms | MMP14 (N-term) |
| Clonality | Polyclonal |
| Preparation | This MMP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the N-terminal region of human MMP14. |
| Concentration (lot specific) | 1.280 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP14 seems to specifically activate progelatinase A, and may thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Expression is significant in stromal cells of colon, breast, and head and neck.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Pig
Other Names
MMP14; Matrix metalloproteinase-14; MMP-X1; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase
NCBI Accession #
NP_004986.1
Other Accession #
Q10739; Q9XT90; P53690; Q9GLE4; NP_004986
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
65894
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IHC: 1:50-100
 | MMP14 Antibody (R160) (Cat# 102-16674) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MMP14 antibody detected the MMP14 protein (arrow). |
 | Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.