Synonyms
FP17780 antibody, MPD antibody, MVD antibody, MDDase antibody, diphosphomevalonate decarboxylase antibody, mevalonate pyrophosphate decarboxylase antibody, mevalonate pyrophosphate decarboxylase antibody, mevalonate (diphospho) decarboxylase antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
43 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 156 and 371 of MVD (Uniprot ID# P53602)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using MVD(107-10962) antibody at 1:100 dilution.

MVD antibody detects MVD protein by Western blot analysis.
A. 30 µg 293T whole lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg A375 whole cell lysate/extract
10 % SDS-PAGE
MVD antibody (107-10962) dilution: 1:1000

Sample (50 ug of whole cell lysate)
A: mouse Liver
10% SDS PAGE
107-10962 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MVD(107-10962) antibody at 1:200 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.