MX1 antibody [N2C2], Internal, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 4599 |
| Protein Name / Synonyms | MX1, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
IFI-78K antibody, IFI78 antibody, MX antibody, MxA antibody, MX1 antibody, myxoma resistance protein 1 antibody, interferon-regulated resistance GTP-binding protein MxA antibody, interferon-induced GTP-binding protein Mx1 antibody, "myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse) antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
76 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 194 and 475 of MX1 (Uniprot ID# P20591)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using MX1(107-10907) antibody at 1:100 dilution.
MX1 antibody [N2C2], Internal detects MX1 protein by Western blot analysis.
A. 30 µg NCI-H929 whole cell lysate/extract
7.5% SDS-PAGE
MX1 antibody [N2C2], Internal (107-10907) dilution: 1:1000
A. 30 µg NCI-H929 whole cell lysate/extract
7.5% SDS-PAGE
MX1 antibody [N2C2], Internal (107-10907) dilution: 1:1000
MX1 antibody [N2C2], Internal detects MX1 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human MX1-transfected 293T cells.
7.5 % SDS-PAGE
MX1 antibody [N2C2], Internal (107-10907) dilution: 1:10000
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human MX1-transfected 293T cells.
7.5 % SDS-PAGE
MX1 antibody [N2C2], Internal (107-10907) dilution: 1:10000
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using MX1(107-10907) antibody at 1:500 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.