Rabbit Anti-Human NANOG (S285) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9H9S0 |
| Gene Id | 79923 |
| Gene Symbols | NANOG |
| Protein Name / Synonyms | NANOG (S285) |
| Clonality | Polyclonal |
| Preparation | This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-292 amino acids from human NANOG. |
| Concentration (lot specific) | 0.310 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Specificity
Other Names
NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog
NCBI Accession #
NP_079141.2
Other Accession #
Q6NSW7; NP_079141
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
34620
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | Western blot analysis of NANOG (arrow) using rabbit NANOG Antibody (S285) (Cat# 102-12699). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies). |
 | Western blot analysis of NANOG Antibody (S285) (Cat# 102-12699) in HepG2 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab. |
 | Fluorescent confocal image of SY5Y cells stained with 102-12699 NANOG (S285) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with 102-12699 NANOG (S285) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.