Synonyms
FLJ11291 antibody, HEM2 antibody, KIAA0587 antibody, MGC8981 antibody, NAP1 antibody, NAP125 antibody, NCKAP1 antibody, membrane-associated protein HEM-2 antibody, nck-associated protein 1 antibody, p125Nap1 antibody, NAP 1 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
129 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 693 and 936 of NCKAP1 (Uniprot ID# Q9Y2A7)
Applications
Applications Tested
Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10546 diluted at 1:1000

NCKAP1 antibody [C1C2], Internal detects NCKAP1 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
5 % SDS-PAGE
NCKAP1 antibody [C1C2], Internal (107-10546) dilution: 1:1000

Sample (30µg whole cell lysate)
A:Hep G2
7.5% SDS PAGE
107-10546 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.