NDUFA10 antibody [N1C3], 0.82 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 4705 |
| Protein Name / Synonyms | NDUFA10 |
| Concentration (lot specific) | 0.82 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CI-42KD antibody, CI-42k antibody, FLJ21923 antibody, MGC5103 antibody, NDUFA10 antibody, complex I 42kDa subunit antibody, "NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial antibody", NADH-ubiquinone oxidoreductase 42 kDa subunit antibody, "NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
41 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 43 and 355 of NDUFA10 (Uniprot ID# O95299)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
NDUFA10 antibody [N1C3] detects NDUFA10 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: NDUFA10 protein stained by NDUFA10 antibody [N1C3] (107-10365) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: NDUFA10 protein stained by NDUFA10 antibody [N1C3] (107-10365) diluted at 1:500.
Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin-embedded colon , using NDUFA10(107-10365) antibody at 1:100 dilution.
Sample(30 µg of whole cell lysate)
A:H1299
B:Raji
10% SDS PAGE
107-10365 diluted at 1:500
A:H1299
B:Raji
10% SDS PAGE
107-10365 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.