NDUFA12 antibody [N1C3], 0.89 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 55967 |
| Protein Name / Synonyms | NDUFA12 |
| Concentration (lot specific) | 0.89 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
B17.2 antibody, DAP13 antibody, NDUFA12 antibody, complex I B17.2 subunit antibody, CI-B17.2 antibody, complex I-B17.2 antibody, CIB17.2 antibody, 13 kDa differentiation-associated protein antibody, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 antibody, NADH-ubiquinone oxidoreductase subunit B17.2 antibody, 13kDa differentiation-associated protein antibody, NADH: ubiquinone oxidoreductase antibody, "NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 12 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
17 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 145 of NDUFA12 (Uniprot ID# Q9UI09)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HepG2, using NDUFA12(107-10850) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA12(107-10850) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: mouse liver
15% SDS PAGE
107-10850 diluted at 1:1000
A: mouse liver
15% SDS PAGE
107-10850 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: Hep G2
12% SDS PAGE
107-10850 diluted at 1:1000
A: Hep G2
12% SDS PAGE
107-10850 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.