NDUFB5 antibody, 0.44 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 4711 |
| Protein Name / Synonyms | NDUFB5 |
| Concentration (lot specific) | 0.44 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CISGDH antibody, DKFZp686N02262 antibody, FLJ30597 antibody, MGC111204 antibody, MGC12314 antibody, SGDH antibody, NDUFB5 antibody, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial antibody, complex I-SGDH antibody, NADH-ubiquinone oxidoreductase SGDH subunit antibody, complex I SGDH subunit antibody, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
22 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 126 and 189 of NDUFB5 (Uniprot ID# O43674)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using NDUFB5(107-10990) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using NDUFB5(107-10990) antibody at 1:500 dilution.
NDUFB5 antibody detects NDUFB5 protein by Western blot analysis.
A.30 µg 293T whole cell lysate/extract
12 % SDS-PAGE
NDUFB5 antibody (107-10990) dilution: 1:1000
A.30 µg 293T whole cell lysate/extract
12 % SDS-PAGE
NDUFB5 antibody (107-10990) dilution: 1:1000
NDUFB5 antibody detects NDUFB5 protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
12 % SDS-PAGE
NDUFB5 antibody (107-10990) dilution: 1:1000
A. 50 µg mouse brain lysate/extract
12 % SDS-PAGE
NDUFB5 antibody (107-10990) dilution: 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.