NDUFS4 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 4724 |
| Protein Name / Synonyms | NDUFS4 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
AQDQ antibody, CI-18 antibody, NDUFS4 antibody, CI-AQDQ antibody, CI-18 kDa antibody, "NADH-coenzyme Q reductase, 18-KD antibody", mitochondrial respiratory chain complex I (18-KD subunit) antibody, complex I 18kDa subunit antibody, complex I-AQDQ antibody, NADH dehydrogenase (ubiquinone) iron-sulfur protein 4 antibody, complex I-18 kDa antibody, "NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial antibody", NADH-ubiquinone oxidoreductase 18 kDa subunit antibody, "NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase) antibody"
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
20 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 175 of NDUFS4 (Uniprot ID# O43181)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using NDUFS4(107-10544) antibody at 1:100 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
107-10544 diluted at 1:1000
A: Mouse brain
12% SDS PAGE
107-10544 diluted at 1:1000
NDUFS4 antibody detects NDUFS4 protein by Western blot analysis.
A. 50 µg Rat heart lysate/extract
12% SDS-PAGE
NDUFS4 antibody (107-10544) dilution: 1:1000
A. 50 µg Rat heart lysate/extract
12% SDS-PAGE
NDUFS4 antibody (107-10544) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded OVCA, using NDUFS4(107-10544) antibody at 1:100 dilution.
Sample(30µg whole cell lysate)
A: 293T
12% SDS PAGE
107-10544 diluted at 1:1000
A: 293T
12% SDS PAGE
107-10544 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.