Rabbit Anti-Human NDUFS4 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O43181 |
| Gene Id | 4724 |
| Gene Symbols | NDUFS4 |
| Protein Name / Synonyms | NDUFS4 (C-term) |
| Clonality | Polyclonal |
| Preparation | This NDUFS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-160 amino acids from the C-terminal region of human NDUFS4. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, FC, IHC-P |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NDUFS4 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase(Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane.
Specificity
Other Names
NDUFS4; NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial; Complex I-18 kDa; Complex I-AQDQ; NADH-ubiquinone oxidoreductase 18 kDa subunit
NCBI Accession #
NP_002486.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
20108
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of NDUFS4 Antibody (C-term) (Cat# 102-17025) in HL-60 cell line lysates (35ug/lane). NDUFS4 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with NDUFS4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of ATDC5 cells using NDUFS4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of NDUFS4 (arrow) using rabbit polyclonal NDUFS4 Antibody (C-term) (Cat# 102-17025). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDUFS4 gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.