Synonyms
CI-23k antibody, CI23KD antibody, TYKY antibody, NDUFS8 antibody, complex I-23kD antibody, complex I 23kDa subunit antibody, NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial antibody, NADH-ubiquinone oxidoreductase 23 kDa subunit antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
24 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 210 of NDUFS8 (Uniprot ID# O00217)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

NDUFS8 antibody detects NDUFS8 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
NDUFS8 antibody (107-11109) dilution: 1:500.

NDUFS8 antibody detects NDUFS8 protein at on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
NDUFS8 antibody (107-11109) dilution: 1:500.

NDUFS8 antibody detects NDUFS8 protein at on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
NDUFS8 antibody (107-11109) dilution: 1:500.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
107-11109 diluted at 1:1000

NDUFS8 antibody detects NDUFS8 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
12 % SDS-PAGE
NDUFS8 antibody (107-11109) dilution: 1:1000

Sample (30 ug of whole cell lysate)
A: Hep G2
12% SDS PAGE
NDUFS8 antibody
107-11109 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.