Rabbit Anti-Human Nestin (S1409) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P48681 |
| Gene Id | 10763 |
| Gene Symbols | NES |
| Protein Name / Synonyms | Nestin (S1409) |
| Clonality | Polyclonal |
| Preparation | This Nestin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1389-1416 amino acids from human Nestin. |
| Concentration (lot specific) | 0.25 mg/ml |
| Recommended Applications | FC, IHC-P, Western Blotting, IF |
| Research Area | Neuroscience |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Specificity
Other Names
NES; Nestin
NCBI Accession #
NP_006608.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
177439
Recommended Dilutions
IF: 1:200; WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Fluorescent confocal image of SY5Y cells stained with Nestin (S1409) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-14592 Nestin (S1409) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the Nestin (S1409) immunosignal to the intermediate filaments, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000132688). |
 | Western blot analysis of Nestin Antibody (S1409) (Cat# 102-14592) in HepG2 cell line lysates (35ug/lane). NES (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Nestin Antibody (S1409), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of HepG2 cells using Nestin Antibody (S1409)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage
Store at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots. Prevent freeze-thaw cycles.
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.