Rabbit Anti-Human NFIC (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P08651 |
| Gene Id | 4782 |
| Gene Symbols | NFIC |
| Protein Name / Synonyms | NFIC (Center) |
| Clonality | Polyclonal |
| Preparation | This NFIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-208 amino acids from the Central region of human NFIC. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NFIC recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Specificity
Other Names
NFIC; NFI; Nuclear factor 1 C-type; CCAAT-box-binding transcription factor; Nuclear factor I/C; TGGCA-binding protein
NCBI Accession #
NP_001231931.1;NP_001231933.1;NP_001231934.1;NP_005588.2;NP_995315.1
Other Accession #
NP_995315.1; NP_005588.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
55675
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | NFIC Antibody (Center) (Cat# 102-12833) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NFIC antibody detected the NFIC protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with NFIC Antibody (Center)(Cat# 102-12833). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFIC immunoreactivity is localized to nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.